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Gram Staining (Or Gram's Method) Is An Empirical Method Of Differentiating Bacterial Species Into Two Large Groups (Gram-Positive And Gram-Negative) Based On The Chemical And Physical Properties Of Their Cell Walls. The Gram Stain Is Almost Always The First Step In The Identification Of A Bacterial Organism. While Gram Staining Is A Valuable Diagnostic Tool In Both Clinical And Research Settings, Not All Bacteria Can Be Definitively Classified By This Technique, Thus Forming Gram Variable And Gram Indeterminant Groups As Well. Here We See Basic Glass With Tested Microbe.